Balance-Essentials

L-Tryptophan

Balance_EssentialsDescription
White, powdered, essential amino acid.
Origin
Precursors and biosynthesis made in UK.
Biosynthesis & Extraction
Biosynthesized from a wild type thermoacidophilic bacteria Bacillus acidocaldarius.
Quality Control
A fructose-based defined fermentation medium, containing 6.5 kg m−3 fructose, 3.2 kg m−3 (NH4)2HPO4, and a salt solution, is designed (by investigating the influence of carbon and nitrogen sources and ions) to create the physiological conditions where the regulatory mechanisms in Bacillus acidocaldarius operate weakly. The effects of oxygen transfer are monitored within limited-oxygen transfer, low-oxygen transfer, and medium-oxygen transfer conditions by using the following parameters:
QO/VR = 0.25 and 0.6 vvm and N = 280 and 600 min−1 in V = 3 dm3 bioreactors, at initial pH 5.0 and T = 54 °C.
The fructose, cell, tryptophan, and the other amino acid and organic acid concentrations, the oxygen transfer rate (OTR), oxygen uptake rate (OUR), and oxygen transfer coefficients (KLa) are determined throughout the bioprocess.

L-Tyrosine

Description
White, powdered, nonessential amino acid.
Origin
Precursors and biosynthesis made in UK.
Biosynthesis & Extraction
Biosynthesized from Escherichia coli strain (derived from an L-phenylalanine).
Quality Control
Following start-up inspection, fermentation medium components, and DI water is batched into the fermentor and sterilized for 30 min. Once the contents have cooled down, 60% glucose feed solution is added using a peristaltic pump such that final concentration of glucose in the tank is 18–23 g/L. Once all prescribed operating parameters such as DO, pH, pressure, temperature, rpm, airflow are stabilized, the culture from the shake-flask is added to the fermentor following aseptic transfer protocols. The fermentor is sampled periodically for biomass growth (OD550) and residual glucose. Off-gas composition is monitored to ensure typical CER or OUR are obtained. After about 6–8 h of elapsed fermentation time (EFT) when the OD550 of the culture is within 8–10 or CER in the range of 35–55 mmol/L/h, the entire content of the seed fermentor that is typically between 7 and 8 kg is transferred to the primary 200 L production vessel. This transfer of seed inoculum is done under pressure using steam sterilized closed loop transfer lines. Physical separation of L-Tyrosine crystals from biomass using a decanter type centrifuge, based on the density difference between the solids is then utilized.

L-Glutamine

Description
White, powdered, nonessential amino acid.
Origin
Precursors and biosynthesis made in UK.
Biosynthesis & Extraction
Biosynthesized from coryneform bacterium.
Quality Control
Following start-up inspection, fermentation medium components, and DI water is batched into the fermentor and sterilized for 30 min. Sulphuric acid 125 mg/l, copper sulphate 12mg/l, ferric sulfate lmg g/l, vitamin B 2mg g/l, biotin 2mg/l are utilized. The controlled temperature is 32°C. The PH is controlled on the 24th hour – 7.0 to 7.2. Physical separation of L-Glutamine crystals from biomass using a nanofiltration technology is then utilized.

Choline Bitartrate

Description
White, powdered, water-soluble essential nutrient.
Origin
Precursors and extraction made in UK.
Biosynthesis & Extraction
Extracted using organic solvents by utilizing liquid cation exchange where tetraphenylboron is the cation-exchange group.
Quality Control
The type and total amount of cation in the aqueous phase and the amount of sodium tetraphenylboron in the organic solvent are precisely monitored.

GABA (gamma-Aminobutyric acid)

Description
White, powdered, nonessential amino acid.
Origin
Precursors and biosynthesis made in UK.
Biosynthesis & Extraction
Biosynthesized from Lactobacillus brevis.
Quality Control
Following start-up inspection, fermentation medium components, and DI water is batched into the fermentor and sterilized for 30 min. Temperature is set at set at 28-33°C, pH 5.0, and initial glutamate concentration 260-520 mM. Glutamate is re-supplemented into the bioreactor at 12 h and 24 h, respectively.

Niacin (Vitamin B3)

Description
White, powdered, essential human nutrients.
Origin
Precursors and biosynthesis made in UK.
Biosynthesis & Extraction
Liquid phase oxidation, utilizing nitric acid.
Quality Control
Reaction temperature is monitored at 310 ℃, pressure of 28MPa. Utilizing technologically advanced titanium tube MEP reactor, especially designed to improve product quality.